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Provedor de dados: |
BABT
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País: |
Brazil
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Título: |
Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants
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Autores: |
Khalekuzzaman,Mohammad
Khatun,Marufa
Rashid,Mohammad Harunur
Sheikh,Mominul Islam
Sharmin,Shamima Akhtar
Alam,Iftekhar
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Data: |
2012-06-01
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Ano: |
2012
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Palavras-chave: |
In vitro
Watermelon
Micropropagation
Hybrid vigor
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Resumo: |
The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About 72% of the regenerated plantlets acclimatized successfully and survived in the soil condition.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300002
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Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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Relação: |
10.1590/S1516-89132012000300002
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Formato: |
text/html
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Fonte: |
Brazilian Archives of Biology and Technology v.55 n.3 2012
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Direitos: |
info:eu-repo/semantics/openAccess
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